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BIOHIT Active B12 holotranscobalamin assay
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Active B12 (holotranscobalamin) ELISA (BIOHIT)

The BIOHIT Active B12 (holotranscobalamin) ELISA directly measures holotranscobalamin (holoTC) – the biochemically active form of vitamin B12 – from human serum. This test is well-suited for the screening of patients with a suspected vitamin B12 deficiency, and overcomes the problems associated with traditional testing methods that measure total, rather than just active, vitamin B12 concentration.

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Key Advantages

Simple technology ideal for any laboratory

Suitable for both automated and manual methods

Avoids interference from Intrinsic Factor Blocking Antibody (IFBA), unlike total B12 kits

Effective for resolving inconclusive total vitamin B12 values

Direct active vitamin B12 screening

Active B12 Testing plays a crucial role in accurately diagnosing vitamin B12 deficiency. Unlike the total B12 blood test, which measures both active and inactive forms of vitamin B12, the BIOHIT Active B12 ELISA specifically quantifies holotranscobalamin (HoloTC), the active form that can be utilized by the body. This distinction is significant since cobalamin is an essential nutrient for the proper formation of red blood cells and neurological function.

Vitamin B12 is a water-soluble vitamin, and approximately 70% to 90% of circulating vitamin B12 is in an inactive form, which cannot be used by cells. The BIOHIT Active B12 assay uses a highly specific monoclonal antibody to bind HoloTC, enabling precise measurement. This makes it particularly useful for patients with inconclusive results from traditional total B12 blood tests. The test is reliable and minimises interference from intrinsic factor blocking antibodies, which can often skew results in total B12 assays.

This test is particularly suitable for screening patients suspected of having an active vitamin B12 deficiency and can also confirm the vitamin B12 status in many patients who receive indeterminate results from total vitamin B12 tests.

What is the BIOHIT Active Vitamin B12 ELISA test used for?

The BIOHIT Active Vitamin B12 ELISA test is used to quantitatively determine the active form of vitamin B12, known as holotranscobalamin (HoloTC), in human serum.

How does the test detect the active form of vitamin B12?

The test detects the active form of vitamin B12 by utilising a highly specific monoclonal antibody that binds to HoloTC present in the serum sample during the incubation process.

What are the advantages of using the BIOHIT Active Vitamin B12 ELISA compared to other methods?

The BIOHIT Active Vitamin B12 ELISA offers several advantages over other methods, including its ability to specifically measure the active form of vitamin B12, providing more accurate and clinically relevant results.

What is the clinical significance of measuring active vitamin B12 levels?

Measuring active vitamin B12 levels is clinically significant as it reflects the biologically active portion of vitamin B12 that can be utilised by cells in the body, thus offering valuable information about vitamin B12 status and potential deficiency.

Can the test differentiate between active and inactive forms of vitamin B12 in serum?

Yes, the BIOHIT Active Vitamin B12 ELISA is specifically designed to differentiate and quantify the active form of vitamin B12 (HoloTC) in serum samples.

Can the test be used for screening patients with suspected vitamin B12 deficiency?

Yes, the test is suitable for screening patients suspected of having a vitamin B12 deficiency, providing valuable diagnostic information to guide further evaluation and treatment.

  • ELISA

  • 96 wells

  • Storage: 2-8 °C

  • Serum