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Total 25OH Vitamin D ELISA (BIOHIT)

The BIOHIT Total 25OH Vitamin D ELISA is a quantitative immunoenzymatic assay for the determination of 25-hydroxyvitamins D2 and D3 (25OHD2 and 25OHD3) in serum and plasma, to aid in the diagnosis of vitamin D deficiency, insufficiency or intoxication.

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Key Advantages

Detects 25-hydroxyvitamin D2 and D3

25-hydroxyvitamin D2 is dissociated from plasma proteins

Assay time 165 minutes

Automatable

Reportable range 4.4-123 ng/ml

R>0.97, validated against ID-LC/MS-MS Ghent Reference Measurement Procedure

Quantitative assay for 25-hydroxyvitamin D

The Total 25OH Vitamin D ELISA (BIOHIT) is a precise quantitative assay designed to measure 25-hydroxyvitamins D2 and D3 in serum and plasma, playing a crucial role in diagnosing vitamin D deficiency, insufficiency, or intoxication. This test is vital because the accurate diagnosis of vitamin D status depends on the measurement of both 25OHD2 and 25OHD3, which are essential for understanding how well the body absorbs calcium.

The 25OH vitamin D test is a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). This assay involves adding samples, controls, and calibrators to microtiter plate wells with incubation buffer, which facilitates the binding of 25OH vitamin D to specific antibodies. Following several precise steps, including the introduction of biotin-labelled and streptavidin-labelled compounds, washing, and the addition of a chromogenic substrate (TMB), the test measures the 25OH vitamin D concentration by interpreting the colour intensity, which is inversely proportional to the concentration. This process ensures reliable results through calibration curve interpolation.

What is 25OH vitamin D?

25OH vitamin D, also known as 25-hydroxyvitamin D, is a measure of the total amount of vitamin D in your body. It’s considered the best indicator of vitamin D status.

Why should I test my patients for 25OH vitamin D levels?

A 25OH vitamin D test helps assess a patient’s vitamin D status, which is crucial for bone health, immune function, and overall well-being. It aids in diagnosing deficiencies and guiding appropriate treatment.

How is 25OH vitamin D measured?

It is typically measured through a blood test called the 25OH vitamin D test, which detects the concentration of 25OH vitamin D in the blood.

What are the optimal levels of 25OH vitamin D?

Optimal levels of 25OH vitamin D can vary depending on individual factors and guidelines from different health organisations. Generally, levels between 30-50 ng/mL (nanograms per millilitre) are considered adequate for most people.

What are the symptoms of 25OH vitamin D deficiency?

Symptoms of deficiency may include fatigue, muscle weakness, bone pain, and an increased risk of bone fractures. Severe deficiency can lead to conditions like rickets in children and osteomalacia in adults.

  • ELISA

  • 40 tests

  • Storage: 2-8 °C

  • Serum / Plasma