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BIOHIT Active B12 holotranscobalamin assay
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Active B12 (holotranscobalamin) ELISA (BIOHIT)

The BIOHIT Active B12 (holotranscobalamin) ELISA directly measures holotranscobalamin (holoTC) – the biochemically active form of vitamin B12 – from human serum. This test is well-suited for the screening of patients with a suspected vitamin B12 deficiency, and overcomes the problems associated with traditional testing methods that measure total, rather than just active, vitamin B12 concentration.

Key Advantages

Direct active vitamin B12 screening

Active B12 Testing plays a crucial role in accurately diagnosing vitamin B12 deficiency. Unlike the total B12 blood test, which measures both active and inactive forms of vitamin B12, the BIOHIT Active B12 ELISA specifically quantifies holotranscobalamin (HoloTC), the active form that can be utilized by the body. This distinction is significant since cobalamin is an essential nutrient for the proper formation of red blood cells and neurological function.

Vitamin B12 is a water-soluble vitamin, and approximately 70% to 90% of circulating vitamin B12 is in an inactive form, which cannot be used by cells. The BIOHIT Active B12 assay uses a highly specific monoclonal antibody to bind HoloTC, enabling precise measurement. This makes it particularly useful for patients with inconclusive results from traditional total B12 blood tests. The test is reliable and minimises interference from intrinsic factor blocking antibodies, which can often skew results in total B12 assays.

This test is particularly suitable for screening patients suspected of having an active vitamin B12 deficiency and can also confirm the vitamin B12 status in many patients who receive indeterminate results from total vitamin B12 tests.